FEBS MPST 2009

July 11-16, 2009, Patras, Greece, Conference & Cultural Center, University of Patras

FEBS Advanced Lecture Course

Matrix Pathobiology, Signaling and Molecular Targets

Conference and Cultural Center, University of Patras

11-16 July, 2009

 

Program: Invited Lectures (L), Selected Talks (ST), Posters (P)

 

Saturday, 11 July


14:30          Transportation from Athens Airport to Patras         

17:00-18:30  Registration at the Conference & Cultural Center / Coffee 

 

Chairpersons: D. Heinegård and C.A. Antonopoulos

 

18:30-19:00  Welcome Addresses


S. Koubias, Rector of the University of Patras

N. Karamanos, Chairman of the Organizing Committee, Head of the Department

 

Honorary Medal Awards to Prof. V. Hascall and Prof. R. Iozzo granted by the Rector of the University of Patras

 

Invited Opening Lectures (L1 - L2)

 

19:00-19:40   V. Hascall (Cleveland Clinic, Ohio, USA)

Hyperglycemia, Ηyaluronan and Αutophagy in Dividing Cells

19:40-20:20   R. Iozzo (Thomas Jefferson University, USA)

Novel Proteoglycan Roles in Cancer and Angiogenesis

20:30          Welcome Reception at the Conference & Cultural Center

21:30          Transportation to Hotels

 

Sunday, 12 July


08:30          Transportation from Hotels to Conference & Cultural Center

 

Chairperson/discussion leader: A. Dell

 

• Invited Lectures / Tutorials (L3 - L5)

 

09:00-09:40   A. Dell (Imperial College, UK)

Mass Spectometry: A Powerful Tool for Systems Glycobiology

09:40-10:20   F. Lamari (University of Patras, Greece)

Glycosaminoglycan Structure, Function and Analysis

10:20-11:00   A. Theocharis (University of Patras, Greece)

Biological Roles of Intracellular Proteoglycans

11:00-11:30   Coffee break

 

• Invited Lectures / Tutorials (L6)

 

11:30-12:00   E. Karousou (University of Insubria, Italy)

Ubiquitination: a Post–Translational Modification of Hyaluronan Synthase

 

• Selected talks (ST1-ST4)

12:00-12:20   D.H. Vynios (University of Patras, Greece)

Colorectal Cancer Progression: Lessons from Glycosaminoglycan Metabolism

12:20-12:30   E. Zinellu (University of Sassari, Italy)

Structural Analysis of Plasma Glycosaminoglycans in Patients Undergoing Carotid Endarterectomy

12:30-12:45   P. Vongchan (University of Chiang Mai, Thailand)

Inhibition of Human Tumor Xenograft Growth in Nude Mice by a Novel Monoclonal Anti–HSPG Isolated from Human Liver

12:45-13:00   Z. Gunnur Dikmen (University of Hacettepe, Turkey)

The Effects of Telomerase Inhibitor “GRN163L” on Different Cell Lines


13:00-14:30   • Lunch

14:30-16:00   • Poster Session (I) / Coffee

 

 Chairperson/discussion leader: J. Couchman

 

• Invited Lectures / Tutorials (L7 - L10)

 

16:00-16:40   J. Couchman (University of Copenhagen, Denmark)

Signaling Roles of Cell Surface Proteoglycans in the Regulation of Integrin–Mediated Adhesion

16:40-17:20   S. Johansson (University of Uppsala, Sweden)

Integrin Signalling via PI3K and its Relevance for Cell Survival and Migration

17:20-18:00   Coffee break

18:00-18:40   E. Papadimitriou (University of Patras, Greece)

Interaction of Pleiotrophin with αvβ3 Integrin Determines its Effect on Endothelial and Tumor Cell Migration

18:40-19:20   J.O. Winberg (University of Tromso, Norway)

Functions and Properties of Gelatinase Complexes

 

• Selected talk (ST5)

19:20-19:30   B. Mirkovic (University of Ljubljana, Slovenia)

Regulation of Cathepsin B Activity by 2A2 Monoclonal Antibody

 

19:30            • Dinner/Event

21:30            Transportation to Hotels

  

Monday, 13 July


08:30               Transportation from Hotels to Conference and Cultural Center

 

Symposium 1: Functional ECM Molecules in Health and Diseases

Chairperson/discussion leader: V. Hascall

 

• Invited Lectures (L11 - L14)

 

9:00-9:40        D. Heinegård (University of Lund, Sweden)

Matrix Molecules and their Fragments in Tissue Homeostasis

9:40-10:10      S. Even-Ram  (Hadassah Hebrew University Hospital, Israel)

ECM and Cytoskeletal Aspects in Stem Cell Pluripotency and Early Neural Differentiation

10:10-10:50    R. Tammi  (University of Kuopio, Finland)

Hyaluronan in Tissue Remodeling

10:50-11:30   P. Heldin (Ludwig Institute for Cancer Research, Sweden)

The Role of Hyaluronan-CD44 Interactions in Tumor Progression

 

11:30-23:00    • Lunch 

                        • Guided excursion tour to Olympia and dinner (optional)

 

Tuesday, 14 July


08:30            Transportation from Hotels to Conference and Cultural Center

 

Symposium 2: ECM Signaling and Pathobiology

Chairperson/discussion leader: C-H. Heldin

 

• Invited Lectures (L15- L19)

 

9:00-9:40        C-H. Heldin (University of Uppsala, Sweden)

Signaling via Receptors for PDGF and TGF-beta-Possible Targets in Tumor Treatment

9:40-10:20     A. Moustakas (Ludwig Institute, Sweden)

TGFb Signalling and the Control of Epithelial-Mesenchymal Transition

10:20-11:00    N. Karamanos (University of Patras, Greece)

Modern Pharmaceutical Inhibitors Targeting Invasion and Metastasis in Solid Tumors – Implication of Metalloproteinases and Proteoglycans

11:00-11:30   • Coffee break

11:30-12:10    H. Nagase (Imperial College, UK)

Rational Approaches for Targeting Metalloproteinases in Arthritis

12:10-12:50   G. Murphy (University of Cambridge, UK)

New Aspects of Metalloproteases and Inhibitors in Matrix Pathobiology

 

• Selected talk   (ST6)

12:50-13:10   A. Pintzas  (National Hellenic Reasearch Foundation, Athens, Greece)

Epithelia to Mesenchymal Transition in Colon Cancer: Signatures, Potential Novel Markers and Sensitisation to Therapeutics

 

13:10-14:30    Lunch

14:30-16:00     • Poster Session (II) / Coffee

 

Symposium 3: Metabolic Regulation of ECM Molecules

Chairperson/discussion leader: L. Kjellen

 

• Invited Lectures (L20 – L22)

 

16:00-16:40   L. Kjellen (University of Uppsala, Sweden)

Cellular Design of Heparan Sulfate

16:40-17:20   A. Passi (University of Insubria, Italy)

Metabolic Control of Glycosaminoglycan Synthesis

17:20-18:00    M. Tammi (University of Kuopio, Finland)

Control of Hyaluronan Synthesis by Substrate Availability and HAS Promoter Activation

18:00-18:30     • Coffee break

 

• Selected talks (ST7-ST14)

18:30-18:50   G. Koliakos (University of Thessaloniki, Greece)

Sodium/ Hydrogen Exchanger 1 Dependent Monocyte Attachment and Migration on Extracellular Matrix Proteins in Obesity and Diabetes

18:50-19:00   C. Österholm (University of Bergen, Norway)

EXT1 Influences FGF–2 Signaling and Fibroblast Interactions with the Extracellular Matrix

19:00-19:10    R. Poulsen (University of Oxford, UK)

Protection against Glucocorticoid–Induced ROS Generation and Forkhead–Mediated Damage Response in Human Tenocytes

19:10-19:20   D. Ollitrault (University of Caen, France)

Bone Morphogenetic Protein – 2 (BMP-2) and Hypoxia Enhance the  Expression of the Phenotypic Markers of Human Articular Chondrocytes in Collagen Sponges. Applications for the Cell Therapy of Articular Cartilage

19:20-19:30   M. Clerici (University of Varese, Italy)

AMPK Regulates Hyaluronan Biosynthesis

19:30-19:40    B. Bernert (University of Uppsala, Sweden)

Hyaluronan-CD44 Interactions Play a Key Role in Breast Cancer Cell Invasion and Adhesion to Endothelium

19:40-19:50   A. Kultti (University of Kuopio, Finland)

Cholesterol Depletion by Methyl-β-cyclodextrin Suppresses Hyaluronan Synthesis by Downregulating Hyaluronan Synthase 2 via Inhibition of Akt and Activation of STAT5

19:50-20:00   S.D. Scilabra (Imperial College, London, UK)

Endocytic Pathways of TIMP-3 and N -TIMP-3

20:00          Transportation to Hotels

 

Wednesday, 15 July


08:30  Transportation from Hotels to Conference and Cultural Center           

 

Symposium 4: Glycobiology, Disease Monitoring and Targeting

Chairperson/discussion leader: N. Taniguchi

 

• Invited Lectures (L23 – L27)

9:00-9:40        N. Taniguchi (University of Osaka, Japan)

Role of Glycans in Disease: Biomarker Discovery and Therapeutics

9:40-10:20     P. Rudd (Conway Institute, University College Dublin, Ireland)

Detailed Analysis of Glycosylated Biomarkers Provides Insights into Potential Roles for Glycans in Cancer

10:20-11:00    A.van Kuppevelt (University of Nijmegen, The Netherlands)

Generation and Application of Phase Display – Derived Antibodies to Glycosaminoglycan Domains

11:00-11:30   Coffee break

11:30-12:10   I. Vlodavsky (Cancer and Vascular Biology Center, Israel)

Heparanase: One Molecule with Multiple Functions in Inflammation and Cancer Progression

12:10-12:50   G. Tzanakakis (University of Crete, Greece)

The Role of SLRP in Osteosarcoma Pathogenesis

 

• Selected talk (ST15)

 12:50-13:10  S. Misra (University of South Carolina, Charleston)

Induction of Growth Factors and Cytokines by Hyaluronan-Tumor Cell Signaling Affects Invasiveness of Tumor Cells

 

13:10-14:30    Lunch

 

Chairperson/ discussion leader: A. Hjerpe

 

• Invited Lecture (L28)

14:30-15:10   A. Hjerpe (Karolinska Institute, Sweden)

Malignant Mesothelioma as Model for Studying the Role of PGs in Cell Differentiation and Malignancy

         

• Selected talks (ST16-ST24)

15:10-15:30   I. Kovalszky (University of Budapest, Hungary)

Deletion Mutant of Syndecan-1 Induce Differentiation of Hepatoma Cell Lines; the Role of Ets-1

15:30-15:40   C. Escrevente (ITQB, Laboratory of Glycobiology, Portugal)

Role of N- Glycosylation on ADAM10 Processing, Activity and Sorting to Exosomes

15:40-15:50   L. Pettinari (University of Milan, Italy)

Increased Extracellular Matrix Remodelling is Induced in Astrocytes by Glioma/ Astrocytes Interaction in a Co-Culture Experimental Model

15:50-16:00   A. Skliris (University of Patras, Greece)

Serglycin Interacts with C1q Subunit of the First Complement Component and Inhibits the Classical Pathway

16:00-16:10   M. Paveliev (University of Helsinki, Finland)

A High Throughput Microscopy System for Studying the CSPG-Dependent Inhibition of Axonal Growth

16:10-16:20   O. Boubriak (University of Oxford, UK)

Oxygen Consumption and Cytochrome c Oxydase Expression in Bovine Nucleus Pulposus Cells Expanded in Monolayer and  Returned to 3-D Environment

16:20-16:30   A.V. Shakhabazau (Institute of Genetics and Cytology, Belarus)

Role of Extracellular Microenviroments in Neural Induction of Human Mesenchymal Stem Cells

16:30-16:40   M. Koutsioumpa (University of Patras, Greece)

A Role of Nucleolin in the ανβ3 Integrin-Mediated Pleiotrophin-Induced Cell Migration

16:40-17:50   H. Pratsinis (NCSR Demokritos Athens, Greece)

Proliferative Response of Human Intervertebral Disc Cells to Exogenous and Autocrine Growth Factors: Involvement of Pivotal Signaling Pathways

20:00              • Dinner at Restaurant "Agnantema" in Rion port

23:00           Transportation to Hotels

 

Thursday, 16 July


08:30  Transportation from Hotels to Conference and Cultural Center 

 

Symposium 5: Signaling and Molecular Targeting

Chairperson/discussion leader: D. Kletsas

 

• Invited Lectures (L29 – L31)

09:00-09:40   L. Schaefer (University of Frankfurt, Germany)

SLRP- Signaling in Inflammation and Fibrosis

09:40-10:20   D. Kletsas (NCSR Demokritos Athens, Greece)

Cellular Senescence and Tissue Homeostasis

10:20-11:00   S. Ghatak (MUSC, Charleston, USA)

CD44 in Cancer Progression

 

• Selected talks (ST25-ST26)

11:00-11:10   P. Dedes (University of Patras, Greece)

The effects of Zoledronate on Tumour Growth and the Multistep Process of Metastasis in Human Breast Cancer Cells

11:10-11:20   V. Gioni (NCSR “Demokritos”, Greece)

Imatinib Mesylate Inhibits the Proliferation and Collagen Synthesis in Human Breast Stromal Fibroblasts

 

11:20-12:00   • Farewell Party

12:00           • Transportation from Patras to Athens Airport

The poster session represents a vital part of the FEBS-MPST 2009 Course. All abstracts, whether or not selected for oral presentations, will be on display as posters during the entire course to provide ample time for informal discussions among students, participants and lecturers. Two poster sessions and poster discussion groups (Sunday 12 and Tuesday 14) have been planed (see scientific program).

P27
Hyaluronidases in colorectal cancer tissue samples
I. Tsouros1, E. Bouga1, D. Bounias2, D. Kyriakopoulou2, M. Stavropoulos2, N. Papageorgakopoulou1, D. A. Theocharis3, D. H. Vynios1
1. Laboratory of Biochemistry, Department of Chemistry, 2. Department of General Surgery, University Hospital, 3. Laboratory of Biological Chemistry, Department of Medicine, University of Patras, Greece

P28
A physical complex between PDGFRβ and TGFβRI involves CD44
Helena Porsch, Carl-Henrik Heldin and Paraskevi Heldin
Ludwig Institute of Cancer Research (Box 595, 751 24 Uppsala, Sweden)

P29
Modified LDL modulate human aortic smooth muscle cells hyaluronan synthesis and motility
B.Bartolini1, M.Viola1,2, D.Vigetti1, E.Karousou1, P.Moretto1, A.Genasetti1, M.Clerici1, G.De Luca1, A.Passi1
1Dipartimento di Scienze Biomediche Sperimentali e Cliniche, Università degli Studi dell’Insubria, Via J.H. Dunant 5, 21100 Varese, Italy, 2Klinik und Poliklinik für Frauenheilkinde und Geburtshilfe Domagkstrasse 11, 48149 Münster, Germany

P30
In search for genetic modifiers that predispose Thin Basement Membrane Nephropathy patients to Focal Segmental Glomerulosclerosis and renal failure
Konstantinos Voskarides1, Alkis Pierides2, Constantinos Deltas1
1Department of Biological Sciences, University of Cyprus, 2Ippokrateon Hospital, Nicosia, Cyprus

P31
Effects of transcriptional factors hc-krox and nf-kb on the regulation of type i collagen expression in human normal and scleroderma fibroblasts
Beauchef Gallic1, Bigot Nicolas1, Kypriotou Madeleine1, Renard Emmanuelle1, Porée Benoît1, Jolivet Muriel2, Widom Russel L3, Oddoss Thierry4, Bordat Patrick4, Demoor Magali1, Boumediene Karim1 and Galéra Philippe1
1Laboratory of Extracellular Matrix and Pathology, EA 3214, IFR 146 ICORE, Faculty of Medicine, University of Caen/Basse Normandie, CHU level 3 14032 Caen cedex, 2Department of Reconstructive Surgery, Saint-Martin Clinic, 14000 Caen, 3Arthritis Center, Boston University School of Medicine, Boston, MA, USA, 4Johnson&Johnson Consumer France, R&D Europe, Campus de Maigremont, 27100 Val de Reuil

P32
The prognostic value of CD147/EMMPRIN is associated with monocarboxylate transporter 1 co-expression in gastric cancer
C. Pinheiro1, A. Longatto-Filho1,2, K. Simões3, C.E. Jacob4, C.J.C. Bresciani4, B. Zilberstein4, I. Cecconello4, V.A.F. Alves3, F. Schmitt5,6, F. Baltazar1
1Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal, 2Instituto Adolfo Lutz, São Paulo, Brazil, 3Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil, 4Department of Gastroenterology, Faculty of Medicine, University of São Paulo, São Paulo, Brazil, 5IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portuga, 6Medical Faculty of the University of Porto, Porto, Portugal

P33
Analysis of raf kinase inhibitor protein (RKIP) expression in GISTs
O. Martinho1, A.Gouveia2,3,5, P.Silva2,5, A.Pimenta2,3,5, RM. Reis1, JM.Lopes2,4,5
1Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, Braga, Portugal. 2IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal. 3Department of Surgery, 4Department of Pathology, H.S. João, 5Medical Faculty of Porto University, Porto, Portugal

P34
Screening of COL4A5 in Hellenic families from Greece and Cyprus with X-linked Alport syndrome
P. Demosthenous1, K. Voskarides1, E. Dafnis2, K. Stylianou2, A. Pierides3, E. Alexopoulos4†, E. Liakou4, P. Giamalis4, I. Tzanaki5, E. Georgaki6, C. Stavrou7, C. Deltas1
1Department of Biological Sciences, University of Cyprus, 2Department of Nephrology, University of Crete, Heraklion, Crete, Greece, 3Department of Nephrology, Ippokrateion Hospital, Nicosia, Cyprus, 4Nephrology Clinic, Faculty of Medicine, Aristotle University of Thessalloniki, Greece, 5Department of Nephrology, General Hospital of Chania, Chania, Greece, 6Department of Pediatric Nephrology, Agia Sophia Childrens Hospital, Athens, Greece, 7Royal Artemis Medical Center, Pafos, Cyprus

P35
Lumican affects actin cytoskeletal organization and human colon cancer cells migration abilities
Agata Radwanska1, Dagmara Baczynska1, Agata Gajda1, Yanusz Wegrowski2, Maria Malicka-Blaszkiewicz1
1Department of Cell Pathology, Faculty of Biotechnology, University of Wroclaw, ul. Przybyszewskiego 63/77, Wrocław 51-148, Poland, 2Laboratory of Medical Biochemistry and Molecular Biology, CNRS UMR 6198, Department of Medicine, University of Reims-Champagne-Ardenne, France

P36
A novel method to visualize molecular structure at the synaptic level after epileptic seizures
Zs. Szepesi1,2, R. Nyilas1, E. Takács1, P. Baracskay1, N. Szilágyi1, A. Czurkó1, G. Juhász1, Andrzej Szczepankiewicz J. Wlodarczyk2, G. Wilczyński2, L. Kaczmarek2
1Laboratory of Proteomics, Institute of Biology, Eötvös Loránd University, Hungary, 2Laboratory of Molecular Neurobiology, Nencki Institute of Experimental Biology, Poland

P37
Selection of internalizing antibodies targeting endothelial cells involved in angiogenesis
O. A. Mandrup, B.F.C. Clark, P. Kristensen
University of Aarhus, Institute of Molecular Biology,Denmark

P38
Potential regulatory roles of extracellular matrix protein fibronectin and galectin 4 in (re)myelination
M. Stancic1, Joachim H. Gabius2, Dick Hoekstra1 and Wia Baron1
1 University Medical Center Groningen, Membrane Cell Biology, Groningen, Netherlands, 2 Ludwig-Maximilians University, Institute of Physiological Chemistry, Munich, Germany

P39
Identification of changes in gene loci potentially associated with epithelial tumors by novel technique of NotI-microarrays
D.D. Ugryn1,3, І.Ya. Skrypkina1, V.V. Gordiyuk1, E.R. Zabarovsky2, V.І. Kashuba1,2, A.V. Rynditch1
1Institute of Molecular Biology and Genetics, Kyiv, Ukraine; 2Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden; 3Kyiv National Taras Shevchenko University, Biochemistry Department, Ukraine

P40
Metabolism of Intervertebral Disc Explants in the Presence of a Fibronectin Fragment
Ying Cui, Jing Yu, Jill Urban
Department of Physiology, Anatomy and Genetics, University of Oxford, Le Gros Clark Building, South Parks Road, Oxford, OX1 3QX, United Kingdom

P41
Evaluation of anti-leukemic potential a benzo[c]phénanthridine: The Ethoxyfagaronine
Ouchani F1, Devy J1, Salesse S1, Helesbeux J J2, Vanquelef E2, Duval O2, Martiny L1, Charpentier E1
(1)CNRS UMR 6237, IFR 53 Biomolécules, Laboratoire Signalisation et Recepteurs Matriciels, Moulin de la Housse, BP 1039, 51687 Reims Cedex 2, France, (2)Laboratoire SONAS, UFR des Sciences Pharmaceutiques et Ingénierie de la Sante, 49100 Angers, France

P42
Modulation of Hyaluronan Synthasis (HASs) activity during cellular sorting.
Anna Genasetti1 , Davide Vigetti1, Evgenia Karousou1, Manuela Viola1, Moira Clerici, Barbara Bartolini1, Giancarlo De Luca1, Hascall VC2, Alberto Passi1
1Dipartimento di Scienze Biomediche Sperimentali e Cliniche, Università degli Studi dell’Insubria, via J.H. Dunant 5, 21100 Varese, Italy; 2Department of Biomedical Engineering Cleveland Clinic Foundation, Cleveland, Ohio, 44195 USA

P43
Parathyroid hormone fragments differentially regulate hyaluronan metabolism and migration ability of MG63 and SaOS2 osteosarcoma cell lines
G. Datsis, A. Berdiaki, D. Nikitovic, G. Chatzinikolaou, G. Chalkiadaki, G. Tzanakakis
Department of Histology, Division of Morphology, School of Medicine, University of Crete, 71110 Heraklion, Greece

P44
TGFB2 and bFGF regulate HSPGs’ expression in fibrosarcoma cell lines to affect their cell adhesion.
A. Kouvidi, D. Nikitovic, H. Syrogianni, E. Fthenou, G. Tzanakakis
Department of Histology, Division of Morphology, School of Medicine, University of Crete, 71110 Heraklion, Greece

P45
AP-1 and TFIID transcription factors are specifically implicated in the regulation of E-cadherin, Integrin and Vimentin gene expression during Epithelial to Mesenchymal Transition
Angeliki Voulgari1, Margarita Kalogeropoulou1, Chrysovalantis Andreolas1, Laszlo Tora2, Irwin Davidson2 and Alexander Pintzas1
1Laboratory of Signal Mediated Gene Expression, Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, 116 35 Athens, Greece
2Institut de Génétique et de Biologie Moléculaire et Cellulaire, 1, rue Laurent Fries, 67404 Illkirch Cedex, France

P46
Characterization of Glycosaminoglycans and Proteoglycans in Human Orthotopic and Heterotopic bone
Vassiliki M. Mania1, Alkiviadis G. Kallivokas2, Christina Malavaki1, Athanasia P. Asimakopoulou1, John Kanakis1, Achilleas D. Theocharis1, George Klironomos2, George Gatzounis2 , Athanasia Mouzaki2, Elias Panagiotopoulos2, Nikos K. Karamanos1
1Department of Chemistry and 2School of Medicine, University of Patras, Greece

P47
Study of the cellular populations exhibiting osteoblast progenitor phenotypes in the peripheral blood of patients and experimental animals following an established head injury (HI)
Kallivokas A., Mouzaki A., Gatzounis G., Sakellaraki P., Klironomos G., Liarou C., Karanikolas M., Karamanos N. and Panagiotopoulos E.
Medical School and Department of Chemistry, University of Patras, Patras, Greece

P48
Serglycin proteoglycan expressed by cancer cell lines and malignant tissues
Korpetinou Angeliki1, Giannopoulou Efstathia2, Marioli Dimitra3, Kalofonos Haralabos2, Papageorgakopoulou Nikoletta1, Theocharis Achilleas1
1Department of Chemistry, Laboratory of Biochemistry, 2Department of Medicine, Laboratory of Clinical Oncology, 3Department of Medicine, Laboratory of General Biology, University of Patras, Greece

P49
Human serum albumin Cys34 oxidative modifications after infiltration in the carotid atherosclerotic plaque
A. J. Lepedda1,2, A. Zinellu2, A. Cigliano1, E. Zinellu1, F. Piredda3, M. A. Casu3, P. P. Bacciu3, G. M. Cherchi1, C. Carru2, M. Formato1
1Dipartimento di Scienze Fisiologiche, Biochimiche e Cellulari, 2Dipartimento di Scienze Biomediche, 3Servizio di Chirurgia Vascolare-Clinica Chirurgica Generale, Università di Sassari, Italy
 
P50
IGF-1Ea and MGF expression in experimentally infarcted rat myocardium: characterization of the mgf e peptide actions in vitro
Stavropoulou A1, Philippou A1, Halapas A1, Sourla A2, Papalois A3, Koutsilieris M1
1Department of Experimental Physiology, MedicalSchool, National Kapodistrian University of Athens, Goudi-Athens,Greece, 2Endo/OncoResearch Medical Laboratories, Ampelokipi, Athens, Greece, 3ELPEN Research Center, Animal Research Facility Unit, Palini, Attiki, Greece

P51
PTHrP and PTH.1R expression in the post-infarction period of rat myocardium
Halapas A1, Philippou A1, Stavropoulou A1, Sourla A2, Papalois A3, Koutsilieris M1
1Department of Experimental Physiology, MedicalSchool, National Kapodistrian University of Athens, Goudi-Athens,Greece, 2Endo/OncoResearch Medical Laboratories, Ampelokipi, Athens, Greece, 3ELPEN Research Center, Animal Research Facility Unit, Palini, Attiki, Greece

P52
Serum cytokine responses following exercise-induced muscle damage in humans
Philippou A1, Halapas A1, Sourla A2, Koutsilieris M1
1Department of Experimental Physiology, Medical School, National and Kapodistrian University of Athens, Goudi-Athens, Greece, 2Endo/oncoresearch Laboratory, Ampelokipi, Athens, Greece

P53
Implication of Epidermal growth factor receptor signaling in growth and metastatic potential of human colon cancer cells
Ch. Gialeli1, D. Kletsas2, N.K. Karamanos1
1Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26110 Patras, Greece; 2Laboratory of Cell Proliferation and Ageing, Institute of Biology, National Center of Scientific Research “Demokritos”, Athens, Greece;

P54
Separation of different mers of hyaluronan by capillary zone electrophoresis
C. J. Malavaki1, E. Mazarakioti1, C. Markellou1, A. Passi2, N.K. Karamanos1
1Department of Chemistry, Laboratory of Biochemistry, University of Patras, 26110 Patras, Greece, 2Department of Experimental and Clinical Biomedical Sciences ,University of Insubria , Varese, Italy

P55
Heparin affects colon cancer cell distribution through p38 mitogen-activated protein kinase
G. Chatzinikolaou, D. Nikitovic, A. Berdiaki, A. Zafiropoulos, G. Chalkiadaki, G.Ν. Tzanakakis
Department of Histology, Division of Morphology, School of Medicine, University of Crete, 71110 Heraklion, Greece

P56
Heparin reduces FAK expression through a JNK-dependent mechanism to regulate melanoma cell adhesion and migration
D. Nikitovic, A. Berdiaki, G. Chalkiadaki, G. Chatzinikolaou, A. Kouvidi, G. Tzanakakis
Department of Histology, Division of Morphology, School of Medicine, University of Crete, 71110 Heraklion, Greece

P57
Qualitative and quantitative analysis of glycosaminoglycans in the anterior lens capsule in patients with and without pseudoexfoliation syndrome
Panos S. Gartaganis,1 Athanasia P. Asimakopoulou,1 Sotirios P. Gartaganis,2 Nikolaos K Karamanos1
1Department of Chemistry, Laboratory of Biochemistry, 2Department of Ophthalmology, Medical School, University of Patras, Greece

P58
Oxidized laminin-1 and collagen IV induce increased monocyte attachment in diabetes mellitus. The role of the alpha2, alphaL and beta2 integrin subunits
Elena Kostidou1,2, George Koliakos2, Konstantinos Paletas3, Martha Kaloyianni1
1Laboratory of Animal Physiology, Department of Zoology, School of Biology, 2Department of Biological Chemistry, Medical School, 3Laboratoty for the Study of Metabolic Diseases, B’ Medical Clinic, Medical School, Aristotle University of Thessaloniki, Thessaloniki 54124, Greece

P59
Increased expression of matrix metalloproteinase-9 and of urokinase plasminogen activator in testicular cancer
E Milia-Argeiti1, E Huet2, B Vallé2, V Labropoulou3, S Menashi2 and A D Theocharis1
1Department of Chemistry, Laboratory of Biochemistry, University of Patras, Greece, CRRET Laboratory, University Paris Est, Creteil, France, 3Department of Medicine, Laboratory of Clinical Oncology, University of Patras, Greece

P60
EMMPRIN/CD147 levels in testicular germ tumor cells in culture do not correlate with their MMP expression
E. Milia-Argeiti1, E. Huet2, B. Vallé2, A. D. Theocharis1 and S. Menashi2
1Department of Chemistry, Laboratory of Biochemistry, University of Patras, Patras, Greece, 2CRRET Laboratory, University Paris Est, CNRS, Creteil, France,
 
P61
Increased activity of β-glucuronidase in bronchoalveolar lavage samples with positive culture in patients with inflammatory lung disease.
E.C. Panagiotopoulou1, S. Fouzas1, I.E. Triantaphyllidou2, K.N. Priftis3, N. Karamanos2, M.B. Anthracopoulos1
1Respiratory Unit, Department of Pediatrics, University of Patras, Greece, 2Department of Chemistry, University of Patras, Greece, 3Department of Allergy-Pneumonology, Penteli Children’s Hospital, P. Penteli, Athens, Greece

P62
Myeloperoxidase and metalloproteinase-9 in bronchoalveolar lavage samples in patients with inflammatory lung disease
E.C. Panagiotopoulou1, S. Fouzas1, O. Kousidou2, I.E. Triantaphyllidou2, K.N. Priftis3, N. Karamanos2, M.B. Anthracopoulos1
1Respiratory Unit, Department of Pediatrics, University of Patras, Greece, 2Department of Chemistry, University of Patras, Greece, 3Department of Allergy-Pneumonology, Penteli Children’s Hospital, P. Penteli, Athens, Greece

P63
Bioanalytic techniques of multiple analysis with the use of microspheres. Basic principles of method and applications
Evagelia Kalimani, Christina Malavaki, N.K. Karamananos
Department of Chemistry, Laboratory of Biochemistry, University of Patras, Greece

P64
FGF-2 mediates melanoma cell adhesion and migration through a FAK dependent mechanism
G. Chalkiadaki, D. Nikitovic, A. Berdiaki, E. Fthenou, G. Chatzinikolaou, G. Tzanakakis
Department of Histology, Division of Morphology, School of Medicine, University of Crete, 71110 Heraklion, Greece

P65
Proteomic analysis of breast cancer cell lines overexpressing the her2/neu oncogene
Vassia Gartaganis1, Leonidas Fragkos-Livanios1, Martina Samiotaki1, Constantin N.Baxevanis2, George Panayotou1
1Protein Chemistry Group, Institute Of Molecular Oncology, BSRC “Al.Fleming”,Vari, Greece, 2Cancer Immunology And Immunotherapy Center, St.Savas Hospital, Athens, Greece

P66
Proteomic analysis of ebv-induced transformation in a human cell line
Zacharati Gkiafi, Martina Samiotaki, George Panayotou
Biomedical Sciences Research Center “Alexander Fleming”, Vari, Greece

P67
Hyaluronic acid as a target molecule in the treatment of asthma and chronic obstructive pulmonary disease
I. Klagas1, G. Karakiulakis1, M. Roth2, E. Papakonstantinou1
12nd Department of Pharmacology, School of Medicine, Aristotle University of Thessaloniki, Greece; 2Pulmonary Cell Research, Dept Research, University Hospital Basel, Switzerland

P68
UV exposure induced changes in hyaluronan, hyaluronan synthase and CD44 stainings of mouse skin
Kari Törrönen1, Hanna Siiskonen1, Timo Kumlin2, Kirsi Rilla1, Markku Tammi1, Raija Tammi1
1Institute of Biomedicine, Anatomy, 2Department of Environmental Science, P.O.Box 1627, 70211 Kuopio, Finland

P69
Preclinical evaluation of the novel kallikrein genes KLK13 and KLK15 as new putative molecular markers for differential diagnosis and suitable therapeutic handling of prostate cancer
Konstantinos Mavridis and Andreas Scorilas
Department of Biochemistry and Molecular Biology, Faculty of Biology, University of Athens, Panepistimiopolis, 15701 Athens, Greece

P70
Development and healing of liver fibrosis in decorin knock out mice
Kornélia Baghy1, Péter Nagy1, Renato V. Iozzo2, Ilona Kovalszky1
11st Department of Pathology and Experimental Cancer Research, Budapest, Hungary, 2Department of Pathology, Anatomy, and Cell Biology, Jefferson Medical College,Thomas Jefferson University, Philadelphia, PA, USA

P71
The Role of nidogens and Laminin-nidogen interaction in basement membrane formation and Function in the skin
M. Bechtel1, S. Mokkapati1, A. Fleger-Weckmann1, M. Reibetanz1, D. Breitkreutz2, N. Smyth3, R. Nischt1
1Department of Dermatology University of Cologne, Germany, 2German Cancer Research Center, Heidelberg, Germany, 3School of Biological Sciences, University of Southampton, UK

P72
Importance of Extracellular matrix protein 1 (ECM1) in maintaining the functional integrity of the human skin
J. Merregaert1, E. Steenackers1 and S. Sercu2
1Laboratory of Molecular Biotechnology, Department of Biomedical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Wilrijk/Antwerp, Belgium, 2EggCentris NV, Researchpark 310, 1731 Zellik, Belgium

P73
The effect of oxidative stress factors in the trabecular cells cytokine production
Mallini P.1 , Topouridou K.1, Koliakos G.1, Empeslidis T.2, Konstas A.2
1Laboratory of Biological Chemistry, 2Ophthalmology Department, AHEPA Hospital, Medical School, Aristotle University of Thessaloniki

P74
Estradiol and genistein as key factors affecting the expression of matrix molecules through estrogen receptors in breast cancer
O. Ch. Kousidou1, A. Berdiaki2, A. Zafiropoulos2, D. Kletsas3, A. Theocharis1, G. N. Tzanakakis2 and N. K. Karamanos1
1 Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26110, Patras, Greece, 2Department of Histology, School of Medicine, University of Crete, Heraklion, Greece, 3Laboratory of cell proliferation and aging, Institute of Biology, N.C.S.R. “Demokritos”, Athens

P75
Mechanisms involved in the Syndecan-1 induced promotion of cancer proliferation and metastasis in HT-1080 cells.
Péterfia B, Szabadkai K, Hollósi P, Paku S, Jeney A, Kovalszky I.
1st Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary

P76
Complement system activation by orthopedic prosthetic particulates
P. Kokkinos1, G. Sfyroera2, M. Markiewski2, A. Vantarakis3, D. Deligianni1
1Laboratory of Biomechanics and Biomedical Engineering, Department of Mechanical Engineering and Aeronautics, University of Patras, University Campus 26504, Rio, Greece, 2Protein Chemistry Laboratory, Department of Pathology and Laboratory Medicine, University of Pennsylvania, School of Medicine, Philadelphia, 401 Stellar-Chance labs 422 Curie Boulevard Philadelphia, PA 19104-6100, USA, 3Laboratory of Hygiene, School of Medicine, University of Patras, University Campus 26504, Rio, Greece

P77
Heparan sulphate chain requirements for syndecan-4 function in cell adhesion
Sandeep Gopal Pakideeri Karat, James R. Whiteford, Atsuko Yoneda, Hinke A.B. Multhaupt and John R. Couchman
Department of Biomedical Sciences, University of Copenhagen, Denmark

P78
Epigenetic silencing of crabp2 and mx1 in head and neck tumors
Calmon, M. F.1; Rodrigues, R. V.2,3; Kaneto, C. M.4; Moura, R. P.5; Silva, S. D.6; Mota, L. D. C.6; Pinheiro, D. G.4; Torres, C.6; Carvalho, A. F.6; Cury, P. M.2; Nunes, F. D.7; Nishimoto, I. N.6; Soares, F. A.6; Silva, A. M. A.8; Kowalski, L. P.6; Brentani, H.6; Zanelli, C. F.9; Silva Jr, W. A.4; Rahal, P.1; Tajara, E. H.2,3; Carraro, D. M.6; Camargo, A. A.5 & Valentini S. R.9
1Department of Biology, IBILCE-UNESP, São José do Rio Preto-SP, 15091-450, Brazil, 2Department of Molecular Biology, School of Medicine-FAMERP, São José do Rio Preto-SP, 15090-000, Brazil, 3Department of Genetics and Evolutionary Biology, Institute of Biosciences-USP, São Paulo-SP, 05508-090, Brazil, 4Department of Genetics, School of Medicine-USP, Ribeirão Preto-SP, 14051-140, Brazil, 5Ludwig Institute for Cancer Research at Oswaldo Cruz Hospital, São Paulo-SP, 01323-903,Brazil, 6A.C. Camargo Cancer Hospital-Medical and Research Center, São Paulo-SP, 01509-900, Brazil , 7School of Dentistry-USP, São Paulo-SP, 05508-000, Brazil, 8Heliópolis Hospital, São Paulo-SP, 04231-030, Brazil, 9Department of Biological Sciences, School of Pharmaceutical Sciences-UNESP, Araraquara-SP, 14801-902, Brazil

P79
Implication of reactive oxygen species in the stimulatory effect of pleiotrophin οn human cell migration
Sotiria Tsirmoula and Evangelia Papadimitriou
Laboratory of Molecular Pharmacology, Department of Pharmacy, University of Patras, Greece

P80
Novel CD44 interacting proteins with possible roles in cell migration and proliferation
Spyros S. Skandalis, Berit Bernert, Carl-Henrik Heldin and Paraskevi Heldin
Ludwig Institute for Cancer Research, Uppsala University, Biomedical Center, Uppsala, Sweden

P81
Pathobiology of patent ductus arteriosus: Hyaluronan signaling
Shibnath Ghatak1, Steven W. Kubalak2, Vincent C. Hascall3, Tammi RH4, Tammi MI4, Amy L. Phelps2, Tim McQuinn2, Stanely Hoffman1, Roger R. Markwald2 and Suniti Misra1
1Cell Biology and Anatomy, and Division of Rheumatology and Immunology (Department of Medicine), Medical University of South Carolina, Charleston, SC 29425, 2Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, 3Department of Biomedical Engineering/ND202, Cleveland Clinic, Cleveland, Ohio, USA, 4Department of Biomedicine/Anatomy, University of Kuopio, PO Box 1627, 70211 Kuopio, Finland.

P82
Changes of syndecan 1 expression in the placenta of patients with preeclampsia and HELLP syndrome.
T. Füle1, K. Karászi1, J. Schönléber1, N. Mihalik1,2, T. Várkonyi2, B. Nagy2, P. Hupuczi2, J. Rigó Jr2, NG Than2, I. Kovalszky1
11st Department of Pathology and Experimental Cancer Research, 21st Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary.

P83
Clinical correlation between bioactive macromolecules and bone biomarkers in breast cancer metastatic bone disease
X. Stachtea1, I. Kanakis1, M. Nikolaou2, D. Pectasides3 and N. K. Karamanos1
1Department of Chemistry, Laboratory of Biochemistry, University of Patras, 26110, Patras; 2Department of Medicinal Oncology, Metaxas Memorial Cancer Hospital, Piraeus; 3Second Propaedeutic Department of Internal Medicine, Athens University Medical School, Attikon University Hospital, Athens, Greece
 
P84
Pproteasome inhibitors enhance the production of metalloproteinases mmp-1 and mmp-3 and inhibit the production of il-6, timp-1 and type-i collagen by nasal polyps fibroblasts
S. D. Athanassiou1, S. Drakouli1, Th. Stathas2, S. Naxakis2, E. Giannopoulou3 and A. J. Aletras1
1Laboratory of Biochemistry, Department of Chemistry, 2Department of Otolaryngology, 3Department of Pharmacology, Medical School, University of Patras, Patras, Greece

P85
Sulfated glycosaminoglycans synthesizing enzymes in colorectal cancer
D. Kalathas1, D. Bounias2, D. Kyriakopoulou2, M. Stavropoulos2, D. A. Theocharis3, N. Papageorgakopoulou1 and D.Vynios1
1Laboratory of Biochemistry, Department of Chemistry, University of Patras, Greece, 2Department of Surgery, University Hospital, Patras, Greece, 3Laboratory of Biological Chemistry, Department of Medicine, University of Patras, Greece

P86
Staphylococcus epidermidis attachment to human endothelial cells is mediated through its 20-kDa PS
Maria I. Krevvata1, Fevronia Kolonitsiou1, Anastasia Spiliopoulou1, Nikos K. Karamanos2, Evangelos D. Anastassiou1
1Department of Microbiology, School of Medicine, 2Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26 500 Patras, Greece

P87
Hyaluronan, hyaluronan synthases and cd44 receptor in colorectal cancer
E. Bouga1, D. Kalathas1, D. Bounias2, D. Kyriakopoulou2, M. Stavropoulos2, N. Papageorgakopoulou1, D. A. Theocharis3, D. H. Vynios1
1Laboratory of Biochemistry, Department of Chemistry, 2Department of Surgery, University Hospital, 3Laboratory of Biological Chemistry, Department of Medicine, University of Patras, 26500 Patras, Greece

P88
Oleuropein prevents the formation of extracellular amyloid β-peptide aggregates and leads to increased production of neuroprotective sAPPa
Myrta Kostomoiri, Apostolia Fragkouli, Marina Sagnou, Leandros Skaltsounis, Mania Pelekanou, Effie Tsilibary and Athina Tzinia
Institute of Pathobiology of Extracellular Matrix, Institute of Biology, NCSR Demokritos, Athens

P89
Hyaluronidases and gelatinases in sera of cancer patients
I.E. Triantaffilidou1, E. Bouga1, I. Tsouros1, K. Kolliopoulos1, D. Bounias2, D. Kyriakopoulou2, M. Stavropoulos2, G. Tsiropoulos3, T. Papadas3, N. Mastronikolis3, N. Papageorgakopoulou1, D. A. Theocharis4, D. H. Vynios1
1Laboratory of Biochemistry, Department of Chemistry, 2Department of General Surgery, University Hospital, 3Department of E.N.T. Surgery, University Hospital, 4Laboratory of Biological Chemistry, Department of Medicine, University of Patras, 26500 Patras, Greece

P90
Comparison of bead-based bioassay and ELISA for the determination of osteocalcin in biological samples
I. Kanakis1, 2, A.V.Noulas2 and N. K. Karamanos1
1Laboratory of Biochemistry, Department of Chemistry, University of Patras, Patras; 2Department of Medical Laboratories, Technological Educational Institute of Larissa, Larissa, Greece

P91
Influence of aromatase and epidermal growth factor receptor inhibitors in non-small cell lung cancer
Ismini Kritikou, Efstathia Giannopoulou, Angelos K. Koutras and Haralabos P. Kalofonos
Clinical Oncology Laboratory, Division of Oncology, Department of Medicine, University Hospital of Patras, Patras Medical School, Rio 26504, Greece

P92
Combined treatment with antiangiogenic and anti-EGFR agents  n glioblastoma
K. Dimitropoulos, E. Giannopoulou, A. Argyriou and H.P. Kalofonos
Clinical Oncology Laboratory, Division of Oncology, Department of Medicine, University Hospital of Patras, Patras Medical School, Rio, 26504, Greece

P93
Mutations in the Discoidin Domain Receptor 2 gene (DDR2) cause the Spondylo-Meta-Epiphyseal Dysplasia, Short Limb-Abnormal Calcification Type syndrome
Annick Raas-Rothschild1, Valerie Cormier-Daire3 , Ziva Ben-Neriah1 , Martine Lemehrer3, Jacob Sosna2, Sarah F Smithson4, Zvi Borochowitz5 ,Ruth Belostotsky6, Ruth Bargal1
1Departments of Human Genetics and 2Radiology, Hadassah Hebrew University Hospital P.O.B 12000, Jerusalem, 91120, Israel, 3Department of Human genetics, Necker-Enfants Malades hospital, Paris, France, 4Department of Clinical Genetics, St Michael's Hospital,Southwell Street,Bristol, BS2 8EG UK.  5Simon Winter Institute for Human Genetics, Bnai-Zion Medical Center, Technion-Faculty of Medicine, POB 4940, Haifa, 31048, Israel, 6Division of Pediatric Nephrology, Shaare Zedek Medical Center

P94
Monitoring chondroitin sulfate in serum of patients with coronary disease
Dimos E. Karangelis1, Athanasia P. Asimakopoulou2, Nikos K Karamanos2, Nikolaos B. Tsilimigas1
1Department of Cardiovascular and Thoracic Surgery, University of Thessaly, Larissa, Greece, 2Department of Chemistry, Laboratory of Biochemistry, University of Patras, Greece

P95
Comparison of fluorophore-assisted cabrohydrate electrophoresis, blyscan assay and capillary electrophoresis in the analysis of chondroitin sulfate
M.-I. Ellina, V. Zafeiropoulou, A.P. Asimakopoulou, Ch. Gialeli, C. Malavaki, N.K. Karamanos
Department of Chemistry, Laboratory of Biochemistry, University of Patras, 26110, Patras, Greece

P96
Crocetin affects the proliferation and expression of metalloproteinases in breast cancer cells MDA-MB-231
Dimitra G. Chryssanthi1, Petros Dedes2, Fotini N. Lamari1, Nikos K. Karamanos2, Paul Cordopatis1
1Laboratory of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, 26500 Patras, Greece, 2Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26500 Patras, Greece

P97
Experimental animal model for chronic cervical myelopathy. Taking advantage of the special characteristics of a synthetic resin
A.G. Mavrakis1, G. Klironomos1, E. Papadaki2, A. Kallivokas4, I.Panagiotopoulos4, N. Karamanos3, I. Kalitsis3, G. Gatzounis1
1Department of Neurosurgery, University of Patras-Greece, 2Department of Anatomy, University of Patras-Greece, 3Department of Chemistry, University of Patras- Greece, 4Department of Orthopedic University of Patras- Greece

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